DNA MEASUREMENT, PROLIFERATION MARKERS, AND OTHER FACTORS IN PITUITARY-ADENOMAS

Citation
A. Kramer et al., DNA MEASUREMENT, PROLIFERATION MARKERS, AND OTHER FACTORS IN PITUITARY-ADENOMAS, Endocrine pathology, 5(4), 1994, pp. 198-211
Citations number
64
Categorie Soggetti
Pathology,"Endocrynology & Metabolism
Journal title
ISSN journal
10463976
Volume
5
Issue
4
Year of publication
1994
Pages
198 - 211
Database
ISI
SICI code
1046-3976(1994)5:4<198:DMPMAO>2.0.ZU;2-L
Abstract
To assess the proliferative activity of pituitary adenomas, 36 surgica lly removed adenomas were studied by light microscopical parameters; m itotic count; expression of PCNA, Ki-67, cathepsin D, and EGF; and ima ge cytometry. Three adenomas (9%) showed high, 11 (34%) medium, 17 (53 %) moderate, and 1 (3%) low structural differentiation. In 10 adenomas (31%), no mitosis was observed. The average was 2.4 mitoses/100 HPF; the highest count was 7.1 mitoses/100 HPF. Eleven adenomas (33.3%) wer e PCNA-negative; in 20 adenomas (60.6%), between 0.05 and 3.9, and in 2 adenomas (6.0%), between 10.5 and 16.4 PCNA-positive nuclei were obs erved. Only a recurrent null-cell adenoma (9%) was Ki-67-negative. Thr ee adenomas (9.1%) were EGF-negative, 28 (84.8%) showed up to 10% posi tive cells, and 2 (6.1%) showed between 10 and 30% positive cells; 19 adenomas (68%) were cathepsin D-negative, including all endocrine-inac tive adenomas. Half the adenomas had an euploid DNA stem line. Endocri ne-inactive adenomas displayed a higher rate of euploid DNA stem lines than endocrine-active adenomas. The S-phase fraction varied between 2 .97 and 28%, with a mean value of 14.4%. Half the adenomas showed an S -phase fraction fo 11.65% or lower.