GLUCOSE SENSORS BASED ON ENZYME IMMOBILIZATION ONTO BIOCOMPATIBLE MEMBRANES OBTAINED BY RADIATION-INDUCED POLYMERIZATION

Amperometric glucose biosensors based on glucose oxidase immobilized o nto poly(2-hydroxyethylmethacrylate) membranes obtained by gamma radia tion-induced polymerization were constructed. In a three-electrode con figuration, smooth or platinized platinum electrodes with different sh apes were used, in order to detect the amount of hydrogen peroxide pro duced in the glucose oxidation. A saturated calomel electrode and a pl atinum foil were used as a reference and counterelectrode, respectivel y. The biocompatible obtained sensors were characterized as regards th e temperature effect, the response, and lifetime. The determination of glucose in standard solutions was carried out, and linear calibration curves were obtained. Depending on the electrode configuration, the s ensor had a response time of 1-4 min, and the measuring range extended from 5 x 10(-5) to 4 x 10(-3)M.