EXTRACTION AND CHEMICAL INVESTIGATION OF KULTHI (MACROTYLONA-UNIFLORUS, LAM) SEED PROTEIN

Studies have been carried out on the protein solubility profile of Kul thi (Macrotylona uniflorus, Lam.) seed in aqueous solution over variou s pHs and at different concentrations of NaCl, Na2SO3, CaCl2, and MgCl 2 at pH 8.0. Amino acid analysis of isolated protein identified 17 ami no acids, 9 of which are essential. Gel-permeation chromatography on S ephadex G-200 revealed the presence of seven components in the protein fraction. Their molecular weights were determined by two comparable s tandard methods. Extractable Kulthi seed proteins in salt solutions we re separated electrophoretically into eight fractions whose molecular weights were found to be 186,200, 131,800, 108,400, 91,200, 53,700, 44 ,700, 38,000, and 27,500.