L3T4(-HELPER CELLS ARE REQUIRED FOR IN-VITRO MATURATION OF IN-VIVO PRIMED T-CELLS IN THE CYTOTOXIC RESPONSE TO MHC CLASS-I DISPARATE CELLS FOLLOWING FOOTPAD IMMUNIZATION() BUT NOT LYT2(+) T)
Oy. Azhipa et Bd. Brondz, L3T4(-HELPER CELLS ARE REQUIRED FOR IN-VITRO MATURATION OF IN-VIVO PRIMED T-CELLS IN THE CYTOTOXIC RESPONSE TO MHC CLASS-I DISPARATE CELLS FOLLOWING FOOTPAD IMMUNIZATION() BUT NOT LYT2(+) T), Immunology letters, 42(3), 1994, pp. 117-122
The induction characteristics of major histocompatibility complex (MHC
) class I-specific cytotoxic T lymphocytes (CTL) after footpad immuniz
ation were studied. Primary CTL were generated in the regional lymph n
odes of C57Bl/6 mice by footpad injection with 10(7) irradiated (2000
rad) spleen cells from MHC class I mutant mouse strain (bm1) followed
by a short in vitro culturing without antigen. The requirement of acce
ssory cells and L3T4(+) T cells during in vitro maturation of in vivo
primed CTL precursor (CTL(p)) was shown. Moreover, using inhibitory an
tibodies, the need for IL-2 and IL-4 for in vitro maturation of CTL wa
s established. We have suggested that accessory cells act at the level
of L3T4(+) T cells which in turn non-specifically up-regulate the CTL
response through the production of growth and differentiation factors
. Thus, the T-helper population of L3T4(+) but not Lyt2(+) phenotype a
ppears to be recruited in the in vitro maturation of in vivo primed CT
L(p) in a given system. Possible mechanisms of this phenomenon are dis
cussed.