IMPROVEMENT OF REPRODUCTIBILITY IN CAPILLARY ZONE ELECTROPHORESIS BY SEQUENTIAL APPLICATION OF HIGH-VOLTAGE AND PRESSURE

Authors
BACHMANN K GOTTLICHER B HAAG I HENSEL W
Citation
K. Bachmann et al., IMPROVEMENT OF REPRODUCTIBILITY IN CAPILLARY ZONE ELECTROPHORESIS BY SEQUENTIAL APPLICATION OF HIGH-VOLTAGE AND PRESSURE, Fresenius' journal of analytical chemistry, 350(12), 1994, pp. 716-717
Citations number
4
Categorie Soggetti
Chemistry Analytical
Journal title
Fresenius' journal of analytical chemistry → ACNP
ISSN journal
09370633
Volume
350
Issue
12
Year of publication
1994
Pages
716 - 717
Database
ISI
SICI code
0937-0633(1994)350:12<716:IORICZ>2.0.ZU;2-T
Abstract
In capillary zone electrophoresis (CZE) problems arise concerning the reproducibility of the measurements. This is due to variations in elec troosmotic flow and analyte velocities. The discontinuous use of high voltage for the separation of analytes and the pressure for the transp ort to the detector offers the possibility to suppress the main causes for these phenomena. The procedure is demonstrated for the separation of 4 phenol derivatives. An increase in reproducibility is found for standard solutions from about 3% to 1%.