DETERMINATION OF ADENINE-NUCLEOTIDES BY FLUORESCENCE DETECTION USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND POSTCOLUMN DERIVATIZATION WITH CHLOROACETALDEHYDE

Authors
STRATFORD MRL DENNIS MF
Citation
Mrl. Stratford et Mf. Dennis, DETERMINATION OF ADENINE-NUCLEOTIDES BY FLUORESCENCE DETECTION USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND POSTCOLUMN DERIVATIZATION WITH CHLOROACETALDEHYDE, Journal of chromatography B. Biomedical applications, 662(1), 1994, pp. 15-20
Citations number
21
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
662
Issue
1
Year of publication
1994
Pages
15 - 20
Database
ISI
SICI code
Abstract
A novel rapid method for the analysis of adenine nucleotides in cells and tissues using post-column derivatisation with chloroacetaldehyde ( CAA) followed by fluorescence detection is described. The CAA is incor porated in the eluent, but only reacts post-column when the temperatur e is elevated to 100 degrees C. Samples are chromatographed following neutralisation of acid extracts. Examples are given using both trichlo roacetic acid extraction for cells in culture, and perchloric acid for murine liver.