SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OFADENOSINE AND DOPAMINE IN RAT STRIATAL TISSUE WITH COMBINED ULTRAVIOLET ABSORBENCY AND ELECTROCHEMICAL DETECTION

A method is described for the simultaneous determination of biogenic a mines, adenosine and their metabolites in rat striatal tissue using hi gh-performance liquid chromatography with ultraviolet spectrophotometr ic and electrochemical detection. Peaks in the chromatograms of striat al tissue extracts were identified by retention times and by on-line a nalysis of peak spectra for adenosine and its metabolites, and by comp aring current ratios of the dual-electrode coulometric detector for mo noamines and metabolites. The assay gives a linear response over the c oncentration range of 0.15-0.60 mu g/ml for biogenic amines, 0.5-2.0 m u g/ml for serotonin, 5-20 mu g/ml for hypoxanthine, adenosine and N-m ethyladenosine, and 10-40 mu g/ml for inosine. The limit of detection for striatal homogenates was 3.5 ng/g for monoamines, 9 ng/g for serot onine, 140 ng/g for hypoxanthine, 290 ng/g for inosine and 80 ng/g for adenosine. The recovery ranged from 88.5% for vanillylmandelic acid t o 110.3% for dopamine. The method was used to measure biogenic amines, adenosine and related metabolites in rat striatal tissues.