HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC AND MEGABORE GAS-LIQUID-CHROMATOGRAPHIC DETERMINATION OF LEVETIRACETAM (UCB L059) IN HUMAN SERUM AFTER SOLID-PHASE EXTRACTION
Tac. Vermeij et Pm. Edelbroek, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC AND MEGABORE GAS-LIQUID-CHROMATOGRAPHIC DETERMINATION OF LEVETIRACETAM (UCB L059) IN HUMAN SERUM AFTER SOLID-PHASE EXTRACTION, Journal of chromatography B. Biomedical applications, 662(1), 1994, pp. 134-139
Citations number
3
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
For monitoring drug levels of the new potential anticonvulsant drug le
vetiracetam (ucb L059) in human serum, two assay methods were develope
d and compared. A solid-phase extraction procedure was followed by eit
her reversed-phase HPLC separation and UV-detection or GLC separation
using cold on-column injection on a megabore column and nitrogen-phosp
horous detection. Absolute recovery of the drug exceeded 97%. Precisio
n and accuracy values for the 16.0 mu g/ml quality control sample were
2.4% and 101 +/- 5% (n = 10), respectively, for the GLC method. Preci
sion and accuracy values for the 12.1 mu g/ml quality control sample w
ere 1.0% and 100 +/- 1% (n = 7), respectively, for the HPLC method. Ag
reement between both methods was excellent (r = 0.993). Both methods a
re suitable for pharmacokinetic studies and therapeutic drug monitorin
g as well. Serum level data for levetiracetam in a patient on chronic
antiepileptic medication are presented.