Tc. Lindholm et al., THE ROLE OF AUTOGENEIC BONE-MARROW IN THE REPAIR OF A SKULL TREPHINE DEFECT FILLED WITH HYDROXYAPATITE GRANULES IN THE RABBIT, International journal of oral and maxillofacial surgery, 23(5), 1994, pp. 306-311
For study of the effect of autogeneic bone marrow on the repair of sku
ll defects filled with hydroxyapatite (HA) granules, 20 trephine defec
ts 11 mm in diameter in 10 New Zealand rabbit skulls were made. Three
defects were implanted with HA granules (HAg), and seven defects were
implanted with HA granules mixed with autogeneic bone marrow (HAg/BM)
from the femoral medullary canal. Autogeneic bone marrow (BM) was impl
anted in three defects, and seven defects were left unfilled. Histomor
phometric quantitation of bone and connective-tissue ingrowth into def
ects showed that the area of new bone ingrowth in BM (70.3+/-8.4%) was
significantly larger than that in HAg (34.4+/-3.9%) and in HAg/BM (24
.0+/-5.1%) (P<0.01 and P<0.01, respectively). Immunohistologic stainin
g detected fibronectin and collagen type III as the main components in
the defects filled with HAg and HAg/BM. The osteoconductive capacity
of HA granules was not stimulated by adding fresh autogeneic bone-marr
ow cells.