ISOLATION, SEQUENCING, AND MUTAGENESIS OF THE GENE ENCODING NAD-DEPENDENT AND GLUTATHIONE-DEPENDENT FORMALDEHYDE DEHYDROGENASE (GD-FALDH) FROM PARACOCCUS-DENITRIFICANS, IN WHICH GD-FALDH IS ESSENTIAL FOR METHYLOTROPHIC GROWTH

Authors
RAS J VANOPHEM PW REIJNDERS WNM VANSPANNING RJM DUINE JA STOUTHAMER AH HARMS N
Citation
J. Ras et al., ISOLATION, SEQUENCING, AND MUTAGENESIS OF THE GENE ENCODING NAD-DEPENDENT AND GLUTATHIONE-DEPENDENT FORMALDEHYDE DEHYDROGENASE (GD-FALDH) FROM PARACOCCUS-DENITRIFICANS, IN WHICH GD-FALDH IS ESSENTIAL FOR METHYLOTROPHIC GROWTH, Journal of bacteriology, 177(1), 1995, pp. 247-251
Citations number
35
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
177
Issue
1
Year of publication
1995
Pages
247 - 251
Database
ISI
SICI code
0021-9193(1995)177:1<247:ISAMOT>2.0.ZU;2-G
Abstract
NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) o f Paracoccus denitrificans;ans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, me thylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrifican s is essential for the oxidation of formaldehyde produced during methy lotrophic growth.