AUTOREGULATION OF AFFERENT ARTERIOLAR BLOOD-FLOW IN JUXTAMEDULLARY NEPHRONS

Utilizing the in vitro blood-perfused juxtamedullary nephron preparati on, we examined the effects of alterations in renal arterial pressure on afferent arteriolar blood flow. With video microscopy and cross-cor relation techniques, arteriolar inside diameters and centerline erythr ocyte velocity were measured to estimate single afferent arteriolar bl ood flow. In response to random changes in perfusion pressure, afferen t arteriolar diameter (n = 8) varied inversely (-0.53 +/- 0.02%/mmHg), and erythrocyte velocity was directly related (1.4 +/- 0.1%/mmHg). Ab ove 95 mmHg, the slope of the relationship between perfusion pressure and afferent arteriolar blood flow did not differ from zero (0.081 +/- 0.053%/mmHg), suggesting efficient autoregulation. When the tubuloglo merular feedback pathway was interrupted by the addition of furosemide (n = 9) or papillectomy (n = 7), there was attenuation of pressure-in duced afferent arteriolar constriction, with impairment in blood flow autoregulation (0.60 +/- 0.05%/mmHg). Superfusion with diltiazem aboli shed autoregulatory responses in afferent arteriolar diameter and bloo d flow (1.5 +/- 0.2%/mmHg). These data demonstrate the autoregulation of blood flow of individual afferent arterioles in juxtamedullary neph rons and suggest that both tubuloglomerular feedback-dependent and -in dependent mechanisms are required for autoregulatory responses.