ELIMINATION OF RAPID POTASSIUM CHANNEL INACTIVATION BY PHOSPHORYLATION OF THE INACTIVATION GATE

The effect of protein kinase C (PKC) on rapid N-type inactivation of K + channels has not been reported previously. We found that PKC specifi cally eliminates rapid inactivation of a cloned human A-type K+ channe l (hKv3.4), converting this channel from a rapidly inactivating A type to a noninactivating delayed rectifier type. Biochemical analysis sho wed that the N-terminal domain of hKv3.4 is phosphorylated in vitro by PKC, and mutagenesis experiments revealed that two serines within the inactivation gate at the N-terminus are sites of direct PKC action. M oreover, mutating one of these serines to aspartic acid mimics the act ion of PKC. Serine phosphorylation may thus prevent rapid inactivation by shielding basic residues known to be critical to the function of t he inactivation gate. The regulatory mechanism reported here may have substantial effects on signal coding in the nervous system.