Sp. Williams et al., MODULATION OF PHORBOL ESTER-INDUCED CONTRACTION BY ENDOGENOUSLY RELEASED CYCLOOXYGENASE PRODUCTS IN RAT AORTA, American journal of physiology. Heart and circulatory physiology, 36(5), 1994, pp. 1654-1662
This study tests the hypothesis that prostaglandins (PGs) released in
response to phorbol esters act as modulators of the phorbol ester-indu
ced smooth muscle contraction. The rate and magnitude of the phorbol 1
2-myristate 13-acetate (PMA)-induced contraction of deendothelialized
rat aorta were decreased by the cyclooxygenase inhibitor, indomethacin
. The thromboxane (Tx) A(2)/PGH(2) receptor antagonist, SQ-29548, also
inhibited PMA-induced contraction, and the magnitude of inhibition wa
s greater than that due to indomethacin. PMA induced the release of PG
I(2), PGE(2), PGF(2 alpha), and arachidonic acid, but not TxA(2). The
amount of PGI(2) released was greater than that of PGE(2) and PGF(2 al
pha). Indomethacin blocked the PMA-induced release of PG, but not of a
rachidonic acid. In PMA-contracted tissues, PGF(2 alpha), PGE(2), and
the stable PGI(2) and PGH(2) analogues, carbacyclin and U-46619, respe
ctively, induced further contraction. Pretreatment of PMA-contracted t
issues with SQ-29548 partially inhibited the PGF(2 alpha)- and PGE(2)-
induced contractions, completely inhibited contraction to U-46619, and
reversed the carbacyclin-induced contraction to relaxation. These res
ults demonstrate that, in rat aorta, PMA induces the release of PGs th
at exert both contractile and relaxant effects but whose net effect is
to accelerate and augment the contraction induced by PMA. The PG-indu
ced increase in PMA contraction is mediated, in large part, through Tx
A(2)/PGH(2) receptor activation. The ability of various PGs, including
carbacyclin, to activate the TxA(2)/PGH(2) receptor suggests that one
or more of these PGs, in addition to, presumably, PGH(2), may be resp
onsible for the increase in PMA contraction. PGI(2) is the only endoge
nously released PG that can account for the relaxant effect.