BINDING OF NONHOMOLOGOUS SERIES OF ANTICANCER DRUGS TO GLUTATHIONE

The interaction of 17 anticancer drugs with reduced glutathione was st udied by charge-transfer reversed-phase thin-layer chromatography and the hydrophobicity, the specific hydrophobic surface area and the rela tive strength of interaction was calculated. Only 7 anticancer drugs s howed significant interaction with glutathione,the relative strength o f interaction depended significantly neither on the hydrophobicity nor on the specific hydrophobic surface area of the drugs. As the interac ting drugs are basic compounds it is reasonable to suppose that their interaction with the acidic glutathione is based on Coulombic forces. The direct binding of these drugs to glutathione makes it probable tha t glutathione may influence the biological activity of the bound antic ancer drugs.