FURTHER STUDY ON THE SPONTANEOUS PARTIAL FOLDING OF CHICKEN HEART APOCYTOCHROME-C

Citation
Jc. Tong et al., FURTHER STUDY ON THE SPONTANEOUS PARTIAL FOLDING OF CHICKEN HEART APOCYTOCHROME-C, Biochemistry and molecular biology international, 34(6), 1994, pp. 1235-1243
Citations number
27
Categorie Soggetti
Biology
ISSN journal
10399712
Volume
34
Issue
6
Year of publication
1994
Pages
1235 - 1243
Database
ISI
SICI code
1039-9712(1994)34:6<1235:FSOTSP>2.0.ZU;2-4
Abstract
It has been previously reported (Biochem. Mol.Biol.Int.,30(5),867-876, 1993) that chicken heart apocytochrome c has stronger tendency to fol d spontaneously in aqueous solution than those from other species, and the relevance of its folding state with its ability to penetrate acro ss the lipid bilayers has also been studied. In the present paper, the spontaneous folding characteristics of chicken heart apocytochrome c was further examined by the studies of Fourier-transform infrared spec tra,the properties of intrinsic tryptophan fluorescence, and the measu rements of hydrophobicity. In addition, it was also demonstrated that the partially-folded apocytochrome c is easily to be aggregated and he nce relatively incompetent for translocation across the phospholipid b ilayers.