NUCLEIC-ACID SEQUENCE AND DNASE HYPERSENSITIVE SITES OF THE 5' REGIONOF THE MOUSE PROGESTERONE-RECEPTOR GENE

To allow study of the control of the promoter of the mouse progesteron e receptor gene and its specific elements, we have isolated nine indiv idual clones and selectively sequenced the 5' region, which includes 5 ' promoter sequence and the non-coding region. Sequence comparison was performed versus the progesterone receptor genes of other species. Mo reover, potential estrogen response elements, progesterone response el ements, and other transcription factor recognition sites were determin ed by sequence analysis. In addition, since it has been confined that chromatin structures are important for regulation of gene expression, we also examined them in different estrogen target organs using the DN ase hypersensitivity assay. A different pattern of the DNase hypersens itivity sites was detected between uterus and mediobasal hypothalamus and these sites were close to specific recognition elements. (C) 1994 Academic Press, Inc.