K. Hagihara et al., NUCLEIC-ACID SEQUENCE AND DNASE HYPERSENSITIVE SITES OF THE 5' REGIONOF THE MOUSE PROGESTERONE-RECEPTOR GENE, Biochemical and biophysical research communications, 205(2), 1994, pp. 1093-1101
To allow study of the control of the promoter of the mouse progesteron
e receptor gene and its specific elements, we have isolated nine indiv
idual clones and selectively sequenced the 5' region, which includes 5
' promoter sequence and the non-coding region. Sequence comparison was
performed versus the progesterone receptor genes of other species. Mo
reover, potential estrogen response elements, progesterone response el
ements, and other transcription factor recognition sites were determin
ed by sequence analysis. In addition, since it has been confined that
chromatin structures are important for regulation of gene expression,
we also examined them in different estrogen target organs using the DN
ase hypersensitivity assay. A different pattern of the DNase hypersens
itivity sites was detected between uterus and mediobasal hypothalamus
and these sites were close to specific recognition elements. (C) 1994
Academic Press, Inc.