M. Hadjisavas et al., PURIFICATION OF A CELL-CELL ADHESION REGULATOR FROM PORCINE SEMINAL-VESICLE FLUID, Biochemical and biophysical research communications, 205(2), 1994, pp. 1206-1216
Seminal plasma derived factors are implicated in mediating inflammatio
n in the female reproductive tract following insemination at mating. D
uring inflammation, leukocytes are activated to express adhesion recep
tors resulting in adherence to each other and for the ECM as well as f
or various cell types. The present study describes the purification of
a leukocyte cell-cell adhesion regulator derived from seminal vesicle
fluid. Seminal vesicle fluid proteins were chromatographed by cation
exchange, hydrophobic interaction and reversed phase. Chromatography o
n Phenyl Superose resolved two distinct forms of cell-cell adhesion re
gulation, type I and II. Reversed phase chromatography of fractions in
ducing type I adhesion resulted in the isolation of a 15kDa adhesion i
nducing protein (pAIF-1). The N-terminal sequence contained a hydropho
bic consensus sequence which exists in: two bovine seminal vesicle pro
teins (BSPA3, PDC 109); IGF-II receptor; fibronectin; and the cation i
ndependent mannose-6-phosphate receptor. (C) 1994 Academic Press, Inc.