REGULATION OF MOUSE UBF GENE BY MULTIPLE GROWTH-RELATED CONTROL ELEMENTS

Transcription of the mouse Upstream Binding Factor (mUBF) gene, that e ncodes one of the essential transcription factors for ribosomal DNA tr anscription, starts from several nucleotides. Neither typical TATA-box nor CCAAT-box is found upstream of the transcription initiation site. In the promoter region, there are eight GC-boxes, eight AP-2 binding consensus sequences, four cAMP response elements, and several serum re sponse element equivalent sequences. These elements appear to play a p ositive role for the regulation of the mUBF gene as a whole. Among ser um response elements, members located between -1182 and -343 are indee d responsive to serum,suggesting their important role in the high expr ession of mUBF under cell growth conditions. (C) 1994 Academic Press, Inc.