7 TRANSMEMBRANE DOMAIN RECEPTOR SUBTYPES IDENTIFIED IN NG108-15 CELLSBY REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION

NG108-15 neuroblastoma x glioma cells are widely used for the study of neurotransmitter receptors. We utilized reverse transcription-polymer ase chain reaction to amplify members of the seven transmembrane domai n class of G-protein linked receptors using RNA isolated from NG108-15 cells. Two complementary DNAs representing receptors were obtained; b ased upon comparison with the sequence database, they probably represe nt the murine dopamine D-1A receptor and a receptor closely related to the serotonin 5HT(1D) receptor subtype. The finding of the 5HT recept or subtype is of interest, as only the 5HT(3) subtype was previously i dentified in NG108-15 cells by pharmacological means. Certain response s of NG108-15 cells to serotonin have been described that do not appea r to be mediated by known 5HT receptor subtypes. The cDNA we cloned ma y therefore represent an additional 5HT(1D) subclass. (C) 1994 Academi c Press, Inc.