H. Inui et al., PARTICIPATION OF DIACYLGLYCEROL KINASE IN MITOGENIC SIGNAL-TRANSDUCTION INDUCED BY PLATELET-DERIVED GROWTH-FACTOR IN VASCULAR SMOOTH-MUSCLECELLS, Biochemical and biophysical research communications, 205(2), 1994, pp. 1338-1344
In rat vascular smooth muscle cells, platelet-derived growth factor (P
DGF) stimulated phosphatidic acid synthesis by activating both of the
two alternative pathways, diacylglycerol kinase (DGK) and phospholipas
e D (PLD). Genistein, a tyrosine kinase inhibitor, inhibited PLD activ
ation but not DGK activation, the latter was inhibited selectively by
R 59022. PDGF-induced DNA synthesis was partially inhibited by geniste
in or R 59022, but these inhibitors had no effect on phorbol ester-ind
uced DNA synthesis. Further, the specific effects of these inhibitors
on PDGF-induced DNA synthesis were additive. (C) 1994 Academic Press,
Inc.