M. Iizuka et al., QUANTITATIVE RT-PCR ASSAY DETECTING THE TRANSCRIPTIONAL INDUCTION OF VASCULAR ENDOTHELIAL GROWTH-FACTOR UNDER HYPOXIA, Biochemical and biophysical research communications, 205(2), 1994, pp. 1474-1480
Quantitative reverse transcription-polymerase chain reaction (RT-PCR)
assay was used to examine the induction of vascular endothelial growth
factor (VEGF) transcript in human osteosarcoma cells, MG-63, under hy
poxic culture condition. Using this assay system, the expression of VE
GF mRNA was estimated eight-fold higher when cells were cultured under
hypoxic condition. Transcription level of hypoxanthine phosphoribosyl
transferase (HPRT) mRNA was also examined as an internal control. HPR
T mRNA level under hypoxia was reduced to one fourteenth. Secretion of
VEGF into the cell culture medium was implied by its stimulating acti
vity on the growth of mouse vascular endothelial cultured cells in vit
ro. (C) Academic press, Inc.