DEVELOPMENT OF IMMOBILIZED ENZYME ENTRAPPED WITHIN INORGANIC MATRIX AND ITS CATALYTIC ACTIVITY IN ORGANIC MEDIUM

A method for entrapment of enzymes within an inorganic matrix was deve loped using a sol-gel process. Such entrapping immobilization of bioca talysts within inorganic matrices has some benefits but it is rather d ifficult to apply in contrast to other types of immobilization and the refore has not been commercialized. The method developed was applied t o entrapping lipase within silica beads. The entrapped lipase was six times more active for esterification in an organic medium than lipase immobilized over silica glass with a binding method which has conventi onally been used. The high activity may come from the characteristics of the present method. It can entrap the enzyme with less denaturation and provide the matrix with physical properties suited to the reactio n, e.g. an abumolance of macro pores.