VIABILITY OF HEMATOPOIETIC PROGENITORS FROM WHOLE-BLOOD, BONE-MARROW AND LEUKAPHERESIS PRODUCT - EFFECTS OF STORAGE MEDIA, TEMPERATURE AND TIME

High-dose cytotoxic chemotherapy can be supported with autologous haem opoietic cells. Cryopreserved bone marrow has conventionally been used for this but blood stem cells are now in common use. We have examined different storage conditions for haemopoietic cells from bone marrow, leukapheresis product and whole blood primed with chemotherapy and fi lgrastim, The mean number of GM-CFC surviving cryopreservation was 80% in leukapheresis product (95% CI 66-96). At 4 degrees C, GM-CFC viabi lity in all three sources of haemopoietic progenitors declined at the same rate, with mean recovery at 24 h of 90% (95% CI 82-98), at 48 h o f 68% (95% CI 61-75) and at 72 h of 47% (95% CI 40-53). Progenitors re mained viable for longer in autologous serum and citrate phosphate dex trose or Iscove's medium than in phosphate buffered saline. There was no significant difference in GM-CFC recovery from whole blood or whole blood buffy layer at 4 degrees C. The capacity to generate and sustai n haemopoiesis in long-term culture is a feature of the more primitive progenitor cells. This capacity was similar in cryopreserved bone mar row and leukapheresis product, cryopreserved or stored for up to 5 day s at 4 degrees C, suggesting that long-term culture-initiating cells a re more resilient than colony-forming cells when cryopreserved or stor ed at 4 degrees C. These data indicate that primed whole blood, in add ition to leukapheresis product and bone marrow, could be stored at 4 d egrees C and used to support multicyclic high-dose chemotherapy.