A 66-KDA PROTEIN ASSOCIATED WITH EPIDERMAL GROWTH-FACTOR RECEPTOR IS A PROTEOLYTIC FRAGMENT OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C

Authors
MEDVEDEVA ND ALEXEYEV VY TSUPKINA NV NIKOLSKY NN
Citation
Nd. Medvedeva et al., A 66-KDA PROTEIN ASSOCIATED WITH EPIDERMAL GROWTH-FACTOR RECEPTOR IS A PROTEOLYTIC FRAGMENT OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C, FEBS letters, 356(2-3), 1994, pp. 299-301
Citations number
18
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
356
Issue
2-3
Year of publication
1994
Pages
299 - 301
Database
ISI
SICI code
0014-5793(1994)356:2-3<299:A6PAWE>2.0.ZU;2-B
Abstract
It is shown that in the A431 cells, EGFR is co-immunoprecipitated with a group of proteins recognized by antibodies to phospholipase C gamma . These are 145- and 47-kDa proteins corresponding to phospholipase C gamma and Nck, respectively, and an unidentified 66-kDa protein. The a ssociation of phosphoinositide-specific phospholipase C gamma and 66-k Da protein to EGFR was observed in the A431 cells with or without the EGF treatment. Trypsin peptide maps of these two proteins are similar so it is assumed that the 66-kDa protein is related to phospholipase C gamma.