SPOT MAPPING ON THE STANDARD PROFILE OF RESTRICTION LANDMARK GENOMIC SCANNING (RLGS) OF SORTED CHROMOSOME-20 USING METHYLATION-INSENSITIVE ENZYME

We established the spot mapping system on a restriction landmark genom ic scanning (RLGS) profile using sorted chromosome as RLGS material. I n this mapping system, we can mapped RLGS spots physically, regardless of their polymorphism, using methylation-insensitive enzymes in all R LGS steps. Here, we report that we identified 28 spots derived from hu man chromosome 20 on an RLGS profile, and that number was in good agre ement with the number predicted from the length of the chromosome 20. (C) Academic Press, Inc.