CLONING, SEQUENCING, AND SITE-DIRECTED MUTAGENESIS OF BETA-LACTAMASE GENE FROM STREPTOMYCES-FRADIAE Y59

Authors
KURAI S URABE H OGAWARA H
Citation
S. Kurai et al., CLONING, SEQUENCING, AND SITE-DIRECTED MUTAGENESIS OF BETA-LACTAMASE GENE FROM STREPTOMYCES-FRADIAE Y59, Antimicrobial agents and chemotherapy, 39(1), 1995, pp. 260-263
Citations number
25
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
39
Issue
1
Year of publication
1995
Pages
260 - 263
Database
ISI
SICI code
0066-4804(1995)39:1<260:CSASMO>2.0.ZU;2-C
Abstract
The beta-lactamase gene from Streptomyces fradiae Y59 was cloned and s equenced. To determine which amino acid residues are critical in bindi ng activity to blue dextran, chimera beta-lactamases were constructed and their binding abilities were determined. The results suggested tha t blue dextran binding may depend more on overall conformation of abou t two-thirds of the beta-lactamase molecule from the N terminus than o n the primary structure.