S. Kurai et al., CLONING, SEQUENCING, AND SITE-DIRECTED MUTAGENESIS OF BETA-LACTAMASE GENE FROM STREPTOMYCES-FRADIAE Y59, Antimicrobial agents and chemotherapy, 39(1), 1995, pp. 260-263
The beta-lactamase gene from Streptomyces fradiae Y59 was cloned and s
equenced. To determine which amino acid residues are critical in bindi
ng activity to blue dextran, chimera beta-lactamases were constructed
and their binding abilities were determined. The results suggested tha
t blue dextran binding may depend more on overall conformation of abou
t two-thirds of the beta-lactamase molecule from the N terminus than o
n the primary structure.