EFFECTIVENESS OF A NEW NON-THROMBOGENIC BIO-ADHESIVE IN MICROVASCULARANASTOMOSES

Negatively-charged fibrin glue was successfully prepared by combining human cryoprecipitate with succinic anhydride. The resulting bio-adhes ive was tested for thrombogenicity and tensile strength by applying it to three groups of Sprague-Dawley rat femoral-artery anastomoses (6 s uture, 2 suture, and no suture anastomoses). Anastomoses were tested b y a standard patency test over 7 days. Both the 6-suture and 2-suture anastomoses with negatively-charged fibrin glue had 100 percent patenc y rates and no pseudoaneurysm formation over 7 days. When positively-c harged fibrin glue was applied to sutured anastomoses, patency rates d ecreased gradually to 50 percent over 7 days. Fibrin glue alone (wheth er negatively- or positively-charged) does not have the tensile streng th to maintain an anastomosis without sutures. However, when applied t o a two-suture anastomosis, the breaking strength of the anastomosis i s more enhanced by the negatively-charged fibrin glue.