ENZYMATIC-HYDROLYSIS OF MONOTERPENE GLYCOSIDES OF PASSION-FRUIT AND MANGO WITH A BETA-GLUCOSIDASE FROM YEAST

Monoterpene glycosides extracted from passion fruit and mango were sub jected to acid hydrolysis, with a phosphoric acid (H3PO4) solution, an d enzymatic hydrolysis using the exocellular beta-glucosidase of Candi da cacaoi. The enzyme was used free and in an immobilized form covalen tly bonded to sodium alginate beads. Following the extraction of free and volatile compounds in the fruit juices by hydrodistillation, the b onded terpenols were hydrolyzed and the free terpenols were recovered by hydrodistillation. The beta-glucosidase enzyme hydrolysis was shown to be an effective process. Immobilized enzyme liberated less terpeno ls than did free enzyme.