AUTOMATED EXTRACTION OF ACETYLGESTAGENS FROM KIDNEY FAT BY MATRIX SOLID-PHASE DISPERSION

A new extraction method for the acetylgestagens medroxyprogesterone ac etate (MPA), chloromadinone acetate and megestrol acetate, from kidney fat, has been developed. The method is a combination of matrix solid phase dispersion and solid phase extraction and is simpler and safer t han previous methods, especially as it can be automated. The recovery was estimated as 59 +/- 5% (mean +/- standard deviation) for MPA. For screening purposes detection can be achieved using a commercially avai lable enzyme immunoassay kit giving detection limits in the range of 1 .0-2.0 ng g(-1).