ENZYME-IMMUNOASSAY FOR THE DETECTION OF LEVAMISOLE IN MEAT AND MILK

Ovine polyclonal antisera were raised to aminolevamisole to develop an enzyme immunoassay for levamisole. The assay has been validated in mi lk and in 100 g l(-1) muscle homogenate. The limit of detection is 1 m u g l(-1). In milk the inter-assay relative standard deviation (s(r)) is 6% at 10 mu g kg(-1) and the intra-assay s(r) value 7.2%. In tissue homogenate the inter-assay s(r) value is 12.7% at 5 mu g kg(-1) and t he intra-assay s(r) value is 3.8%. Total incubation time is 1 h.