DETERMINATION OF AFLATOXIN B-1 IN AGRICULTURAL COMMODITIES BY TIME-RESOLVED FLUOROIMMUNOASSAY AND IMMUNOENZYMOMETRIC ASSAY

Authors
BACIGALUPO MA IUS A MERONI G DOVIS M PETRUZZELLI E
Citation
Ma. Bacigalupo et al., DETERMINATION OF AFLATOXIN B-1 IN AGRICULTURAL COMMODITIES BY TIME-RESOLVED FLUOROIMMUNOASSAY AND IMMUNOENZYMOMETRIC ASSAY, Analyst, 119(12), 1994, pp. 2813-2815
Citations number
16
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst → ACNP
ISSN journal
00032654
Volume
119
Issue
12
Year of publication
1994
Pages
2813 - 2815
Database
ISI
SICI code
0003-2654(1994)119:12<2813:DOABIA>2.0.ZU;2-P
Abstract
A time-resolved fluoroimmunoassay (TR-FIA) and an immunoenzymometric a ssay (IEMA) were applied for the measurement of aflatoxin B-1 in soya seeds, dried figs and raisins. The extraction procedure was simple and no clean-up was found to be necessary. Limits of detection were 0.5 m u g kg(-1) for TR-FIA (range of linearity of calibration graph 2.5-5 x 10(4) pg; inter-assay relative standard deviation s(r) <5%) and 0.2 m u g kg(-1) for IEMA (linear range 1.0(-5) x 10(3) pg; s(r) <11%).