Yc. Cheah et al., NEW MURINE POLYMORPHISMS DETECTED BY RANDOM AMPLIFIED POLYMORPHIC DNA(RAPD) PCR AND MAPPED BY USE OF RECOMBINANT INBRED STRAINS, Mammalian genome, 5(12), 1994, pp. 762-767
Oligonucleotide primers of random sequence that were 12 bases in lengt
h, 58% in GC content, and lacking internal palindromes were designed.
By random amplified polymorphic DNA (RAPD) PCR, these primers were use
d to survey for DNA variations between the progenitors of the mouse AX
E and BXA recombinant inbred sets (A/J and C57BL/6J). We identified 17
DNA variants detected by 10 primers. Map positions for these variants
were determined by comparing their strain distribution patterns in th
e AXE, BXA recombinant inbred sets with strain distribution patterns o
f previously published loci. When necessary, BXD and NXSM recombinant
inbred sets were also used. These 17 new loci mapped to 12 chromosomes
. The 10 primers were also used to survey 20 inbred mouse strains incl
uding the progenitors of other recombinant inbred sets and four mouse
strains recently inbred from the wild (CAST/Ei, MOLF/Ei, PERA/Ei, and
SPRET/Ei).