EXPRESSION OF RAT-BRAIN NITRIC-OXIDE SYNTHASE IN BACULOVIRUS-INFECTEDINSECT CELLS AND CHARACTERIZATION OF THE PURIFIED ENZYME

Authors
HARTENECK C KLATT P SCHMIDT K MAYER B
Citation
C. Harteneck et al., EXPRESSION OF RAT-BRAIN NITRIC-OXIDE SYNTHASE IN BACULOVIRUS-INFECTEDINSECT CELLS AND CHARACTERIZATION OF THE PURIFIED ENZYME, Biochemical journal, 304, 1994, pp. 683-686
Citations number
29
Categorie Soggetti
Biology
Journal title
Biochemical journal → ACNP
ISSN journal
02646021
Volume
304
Year of publication
1994
Part
3
Pages
683 - 686
Database
ISI
SICI code
0264-6021(1994)304:<683:EORNSI>2.0.ZU;2-H
Abstract
Rat brain nitric oxide synthase was expressed to a high level in bacul ovirus-infected insect cells and purified to apparent homogeneity by a ffinity chromatography. The enzyme had a specific activity of similar to 1 mu mol of citrulline.min(-1).mg of protein(-1) and contained 0.93 , 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H(4)biopterin), FAD and FMN per mol of subunit respectively.