DELTA(3),DELTA(2)-ENOYL-COA ISOMERASE IS THE PROTEIN THAT COPURIFIES WITH HUMAN GLUTATHIONE S-TRANSFERASES FROM S-HEXYLGLUTATHIONE AFFINITYMATRICES

An unidentified 30 kDa protein frequently copurifies with human glutat hione S-transferases from S-hexyl-glutathione affinity matrices. Appli cation of two-step sequential affinity chromatographic methods yielded a homogeneous preparation of that protein from human liver specimens. The protein was digested with Achromobacter protease I, and sequences of peptides resolved by h.p.l.c. showed a high degree of identity wit h those of rat mitochondrial Delta(3),Delta(2)-enoyl-CoA isomerase. Th e human protein also exhibited catalytic activity with Delta(3)-cis-oc tenyl CoA as a substrate. Thus it is identified as liver Delta(3),Delt a>(2)-enoyl-CoA isomerase.