Y. Takahashi et al., DELTA(3),DELTA(2)-ENOYL-COA ISOMERASE IS THE PROTEIN THAT COPURIFIES WITH HUMAN GLUTATHIONE S-TRANSFERASES FROM S-HEXYLGLUTATHIONE AFFINITYMATRICES, Biochemical journal, 304, 1994, pp. 849-852
An unidentified 30 kDa protein frequently copurifies with human glutat
hione S-transferases from S-hexyl-glutathione affinity matrices. Appli
cation of two-step sequential affinity chromatographic methods yielded
a homogeneous preparation of that protein from human liver specimens.
The protein was digested with Achromobacter protease I, and sequences
of peptides resolved by h.p.l.c. showed a high degree of identity wit
h those of rat mitochondrial Delta(3),Delta(2)-enoyl-CoA isomerase. Th
e human protein also exhibited catalytic activity with Delta(3)-cis-oc
tenyl CoA as a substrate. Thus it is identified as liver Delta(3),Delt
a>(2)-enoyl-CoA isomerase.