J. Poudrier et T. Owens, CO-STIMULATION BY ANTIIMMUNOGLOBULIN IS REQUIRED FOR B-CELL ACTIVATION BY CD40L(LOW) T-CELLS, European Journal of Immunology, 24(12), 1994, pp. 2993-2999
During cognate B : T interactions, B cells encounter antigen (Ag) thro
ugh surface immuno-globulin (sIg) and present antigenic peptides to T
helper (Th) cells. However, most in vitro systems used to study contac
t events involved in the delivery of T help for B cells circumvent the
requirement for T cell Ag specificity by using anti-CD3/T cell recept
or (TcR) monoclonal antibodies (mAb) to activate T cells. To study the
role of sIg engagement in the responsiveness of B cells to T help, we
pre-treated small resting B cells with soluble anti-kappa mAb prior t
o contact with an activated Th1 clone. By reducing the concentration o
f anti-TcR mAb we obtained low levels of CD40 Ligand (CD40L(low)) on T
h cells, comparable to those expressed by lymph node T cells activated
in vitro (ex vivo T cells). In contrast to untreated B cells, which d
id not respond to CD40L(low) Th, anti-Ig-treated B cells responded str
ongly. Low buoyant density B cells also responded to CD40L(low) Th cel
ls. There was no B cell response to resting Th cells. mAb against CD54
/intercellular adhesion molecule-1 or major histocompatibility complex
(MHC) class II completely inhibited B cell responses to CD40L(low) Th
1 cells, equivalent to the effects of blocking CD40 interactions. This
contrasts with mAb blocking responses to CD40L(high) Th, where CD40 e
ffects predominate. Our data show that sIg engagement is necessary for
the induction of B cell response to CD40L(low) Th cells. Anti-CD3-act
ivated ex vivo T cells that were also CD40L(low) did not provide help
to small resting B cells, but did induce responses from sIg-stimulated
B cells. Thus, our data support a requirement for sIg signaling in ph
ysiological B cell activation, and further confirm previous work showi
ng CD40 ligation to be necessary but not sufficient for delivery of T
help to B cells.