HISTOCHEMICAL-DEMONSTRATION OF MEMBRANOUS LOCALIZATION OF ENDOTHELIALNITRIC-OXIDE SYNTHASE IN ENDOTHELIAL-CELLS OF THE RAT-BRAIN

Authors
TOMIMOTO H AKIGUCHI I WAKITA H NAKAMURA S KIMURA J
Citation
H. Tomimoto et al., HISTOCHEMICAL-DEMONSTRATION OF MEMBRANOUS LOCALIZATION OF ENDOTHELIALNITRIC-OXIDE SYNTHASE IN ENDOTHELIAL-CELLS OF THE RAT-BRAIN, Brain research, 667(1), 1994, pp. 107-110
Citations number
23
Categorie Soggetti
Neurosciences
Journal title
Brain research → ACNP
ISSN journal
00068993
Volume
667
Issue
1
Year of publication
1994
Pages
107 - 110
Database
ISI
SICI code
0006-8993(1994)667:1<107:HOMLOE>2.0.ZU;2-I
Abstract
We examined cellular and subcellular localization of endothelial nitri c oxide synthase by using immunoelectron microscopy. The results showe d that immunoreaction products were associated with membranous structu re, likely the endoplasmic reticulum and cytoplasmic vesicles. These m embranous localization of imunoreactivities at electron microscopic le vel corresponded to the perinuclear granular structures observed by li ght microscopic immunohistochemistry for endothelial nitric oxide synt hase as well as NADPH diaphorase histochemistry.