COMPARISON OF THE BINDING OF NICOTINIC AGONISTS TO RECEPTORS FROM HUMAN AND RAT CEREBRAL-CORTEX AND FROM CHICK BRAIN (ALPHA(4)BETA(2)) TRANSFECTED INTO MOUSE FIBROBLASTS WITH ION-CHANNEL ACTIVITY

Authors
COURT JA PERRY EL SPURDEN D LLOYD S GILLESPIE JI WHITING P BARLOW R
Citation
Ja. Court et al., COMPARISON OF THE BINDING OF NICOTINIC AGONISTS TO RECEPTORS FROM HUMAN AND RAT CEREBRAL-CORTEX AND FROM CHICK BRAIN (ALPHA(4)BETA(2)) TRANSFECTED INTO MOUSE FIBROBLASTS WITH ION-CHANNEL ACTIVITY, Brain research, 667(1), 1994, pp. 118-122
Citations number
27
Categorie Soggetti
Neurosciences
Journal title
Brain research → ACNP
ISSN journal
00068993
Volume
667
Issue
1
Year of publication
1994
Pages
118 - 122
Database
ISI
SICI code
0006-8993(1994)667:1<118:COTBON>2.0.ZU;2-F
Abstract
(-)-Nicotine, cytisine and carbachol evoked Rb-86 efflux from mouse fi broblasts stably transfected with alpha(4) beta(2) chick brain nicotin ic subunits. This response to (-)-nicotine was inhibited by mecamylami ne and dihydro-beta-erythroidine and was mirrored by a rise in intrace llular Ca2+ measured by microspectrofluorimetry. Lobeline and isoareco lone methiodide evoked no significant Rb-86 from cells and unlike the above agonists displayed significantly different IC50 values for the d isplacement of [H-3]nicotine from mammalian (rat and human cerebral co rtex) and transfected fibroblast membranes.