MODIFIED RNA SEQUENCE POOLS FOR IN-VITRO SELECTION

Authors
LIN Y QIU Q GILL SC JAYASENA SD
Citation
Y. Lin et al., MODIFIED RNA SEQUENCE POOLS FOR IN-VITRO SELECTION, Nucleic acids research, 22(24), 1994, pp. 5229-5234
Citations number
43
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
22
Issue
24
Year of publication
1994
Pages
5229 - 5234
Database
ISI
SICI code
0305-1048(1994)22:24<5229:MRSPFI>2.0.ZU;2-8
Abstract
We report the use of modified RNA, in which the 2'-OH group of pyrimid ines is replaced by a 2'-amino (2'-NH2) group to identify high affinit y ligands specific for human neutrophil elastase (HNE) by in vitro sel ection. Compared to unmodified RNA the P'-NH2-modified RNA ligands sho w enhanced stability in human serum and urine. Use of RNase T1 cleavag e data in the presence of K+ and Li+ ions suggests that the modified R NA ligands selected for HNE form an intermolecular G-quartet structure .