IDENTIFICATION OF INSULIN-LIKE PEPTIDES IN CEREBRAL GANGLIA NEUROSECRETORY-CELLS OF THE MUSSEL MYTILUS-EDULIS

The immunostaining patterns of cerebral ganglia sections from the muss el Mytilus edulis with monoclonal antibodies raised against cerebral g anglia (CG) extracts were compared to those obtained with various poly clonal anti-insulin-like antibodies. One of the monoclonal antibodies (MAB 46) revealed clusters of positive cells in localization comparabl e to those revealed by the polyclonal antibodies. The nature of the an tigen recognized by MAB 46 and the polyclonal antibodies was compared by gel filtration-HPLC of a cerebral ganglia extract. Similar peaks we re revealed by the monoclonal and polyclonal antibodies. MAB 46 signif icantly inhibited the cerebral ganglia induced stimulation of amino-ac id incorporation by mantle edge cell suspensions, suggesting that the antigen recognized by MAB 46 is involved in the control of growth.