FAS LIGAND MEDIATES ACTIVATION-INDUCED CELL-DEATH IN HUMAN T-LYMPHOCYTES

A significant proportion of previously activated human T cells undergo apoptosis when triggered through the CD3/T cell receptor complex, a p rocess termed activation-induced cell death (AICD). Ligation of pas on activated T cells by either Fas antibodies or recombinant human Fas-l igand (Fas-L) also results in cytolysis. We demonstrate that these two pathways of apoptosis are causally related. Stimulation of previously activated T cells resulted in the expression of Fas-L mRNA and lysis of Fas-positive target cells. Fas-L antagonists inhibited AICD of T ce ll clones and staphylococcus enterotoxin B (SEB)-specific T cell lines . The data indicate AICD in previously stimulated T cells is mediated by Fas/Fas-L interactions.