H. Saloniemi et al., PHYTOESTROGEN CONTENT AND ESTROGENIC EFFECT OF LEGUME FODDER, Proceedings of the Society for Experimental Biology and Medicine, 208(1), 1995, pp. 13-17
This study is a summary of Finnish investigations of the phytoestrogen
content of legume plants, red clover, white clover, alfalfa, and goat
's rue. In addition to the chemical analyses, biological studies were
performed. Uterine weight of immature rats was used as an indicator of
the estrogenic effect of the fodder used. All red clover varieties st
udied contained estrogenic isoflavones, especially formononetin and bi
ochanin-A. The phytoestrogen content varied from 1.0% to 2.5% of dry m
atter. The biological study of white clover showed a clear estrogenic
effect not visible through chemical analysis. Alfalfa contains small q
uantities of formononetin and biochanin-A, but 25-65 ppm coumestrol in
dry matter. The estrogenic effect of alfalfa was obvious in the biolo
gical study. Goat's rue did not contain any known phytoestrogens, and
the biological study was completely negative.