OLEUROPEIN ON LIPID AND FATTY-ACID COMPOSITION OF RAT-HEART

Male rats of the Wistar strain were given oleuropein for 3 weeks at a dose of 25 or 50 mg/kg of body weight. Heart samples were analyzed for the lipid composition by the Iatroscan TLC/FID technique and for the fatty acid profile of neutral and polar lipids by the capillary gas ch romatography. In addition, the oleuropein, alpha- and beta-tocopherol content in the total heart lipids were also determined. Oleuropein tre atment increased the level of cholesteryl esters, but it decreased the level of triacylglycerols, in the heart of animals. Sphingomyelin and lysophosphatidylcholine levels were only higher in the 50 mg oleurope in group. There was also a significant reduction in the linoleic acid content in the heart polar lipids of oleuropein-treated animals. As co nsequence of treatment, oleuropein was incorporated into the heart of animals in a concentration-dependent manner, whereas the alpha- and be ta-tocopherol content decreased. Oleuropein may therefore act as a sub stitute of the natural antioxidants in heart, with minor changes in th e unsaturated lipids.