Lgmt. Tuinstra et al., THE DUTCH PCB DIOXIN STUDY - DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF DIOXINS, PLANAR AND OTHER PCBS IN HUMAN-MILK, Chemosphere, 29(9-11), 1994, pp. 1859-1875
A method for the determination of dioxins, planar PCBs and other PCBs
in human milk is described. The work is part of a big Dutch project, (
seven institutes participate) in which 400 human milk samples from two
areas (Rotterdam and Groningen) taken at two different times (10 and
42 days) after delivery had to be analysed for planar PCBs, dioxins an
d other PCBs (1st sampling) and only other PCBs (2nd sampling). Planar
compounds are determined with GC/HRMS, other PCBs with GC/ECD. As typ
e of analytes for Ist and 2nd sampling are different the method was fo
cussed on these analytes. After fat extraction, clean up was carried o
ut on GPC and alumina columns. Only in case of analysis of planar comp
ounds and other PCBs, chromatography on porous graphitised carbon was
carried out after which GC-HRMS (planar compounds) and GC/ECD (other P
CBS) was performed; otherwise GC/ECD after alumina clean up was perfor
med. The method is validated through recovery experiments with standar
ds (other PCBs, labelled planar compounds), comparison of results for
other PCBs obtained with the two different clean-up procedures, reprod
ucibility of quality control samples'' for planar compounds and blanc
chemicals.