THE NUCLEOTIDE-SEQUENCE OF THE 5' NON-CODING AND CAPSID CODING GENOMEREGIONS OF 2 BOVINE ENTEROVIRUS STRAINS

The sequence of cDNA clones representing the 5' non-coding regions (NC R) and capsid regions of two bovine enteroviruses (strains PS-87 and R M-2; serotype two viruses) have been determined and compared with that obtained from a serotype one strain (VG-5-27). All three strains show ed a longer 5' NCR compared to human enteroviruses and rhinoviruses du e in part to a hundred residue insertion approximately at a hundred re sidues in from the 5' end. However, another domain occurring at nucleo tide 187-222 in poliovirus is absent in each bovine enterovirus. Compa risons of the predicted structural protein amino acid sequences indica te that PS-87 shares most sequence identity with RM-2 and then with VG -5-27 in that order. The VP1 protein of PS-87 and RM-2 are shorter tha n the equivalent VP1 of VG-5-27 due in part to a truncation at their C -terminii. VP3 is only slightly smaller than VP2 in each virus.