Glycoconjugates from five trypanosomatid genera-Crithidia, Herpetomona
s, Endotrypanum, Leishmania, and Trypanosoma-were extracted with Trito
n X-114 and analyzed by sodium dodecyl sulfate polyacrylamide gel elec
trophoresis followed by periodic acid-Schiff staining. Most of the gly
coconjugates were detected in the hydrophobic phase, indicating the pr
esence of anchored glycoconjugates. All the trypanosomatids expressed
a glycoconjugate with a low molecular weight (below 20 kDa) in this ph
ase. In each species, however, a characteristic and specific pattern o
f glycoconjugates was also observed in both phases. In the hydrophobic
phase: 14-29 kDa glycoconjugates in C. guilhermei; 24-70 kDa in C. fa
sciculata, C. luciliae, E. schaudinni, and T. cruzi Y and G strains; 4
5-66 kDa in C. oncopelti and H. samuelpessoai; above 36 kDa in T. dion
isii; 20-24 kDa, 36-45 kDa, and 70 kDa in L. tarentolae and T. mega. I
n the hydrophilic phase, typical glycoproteins were observed in some t
rypanosomatids: 60 kDa in T. mega and T. cruzi Y strain; 70 kDa in H.
samuelpessoai; 66 kDa in C. oncopelti; 20-70 kDa in C. luciliae, These
findings suggest that Triton X-114-extracted glycoconjugates could be
useful markers for trypanosomatid taxonomy,