AMIPRILOSE HYDROCHLORIDE INHIBITS PORCINE AORTIC SMOOTH-MUSCLE CELL-PROLIFERATION IN-VITRO

The effect of amiprilose hydrochloride, a synthetic monosaccharide, on smooth muscle cell proliferation was studied in vitro. Using porcine aortic smooth muscle cell cultures, amiprilose significantly inhibited cell proliferation in a dose-dependent fashion at concentrations of 0 .5-1.0 mg/ml. This antiproliferative effect was reversible upon removi ng amiprilose by cell washing and adding fresh media, Pretreating cell s with amiprilose before serum stimulation did not affect subsequent g rowth. Inhibition of proliferation occurred when amiprilose was added up to 48 hours after serum stimulation. As such, amiprilose deserves f urther investigation as a potential therapeutic agent in preventing re stenosis after angioplasty.