My. Deng et Do. Cliver, PERSISTENCE OF INOCULATED HEPATITIS-A VIRUS IN MIXED HUMAN AND ANIMALWASTES, Applied and environmental microbiology, 61(1), 1995, pp. 87-91
The persistence of hepatitis A virus (HAV) was determined both in mixt
ures of septic tank effluent (STE) with dairy cattle manure slurry (DC
MS) and in mixtures of STE with swine manure slurry (SMS). HAV was con
sistently inactivated more rapidly in the two types of mixed wastes th
an in STE alone or in the control Dulbecco's phosphate-buffered saline
(PBS). At 5 degrees C, the D values (time, in days, for a 90% reducti
on of virus titer) were 34.6 for the mixed STE and DCMS, 48.5 for the
mixed STE and SMS, 58.5 for STE, and 217.4 for the Dulbecco's PBS cont
rol, At 22 degrees C, the D values were 23.0, 17.1, 35.1, and 90.1 for
the four suspension media, respectively. A comparison of HAV inactiva
tion in mixed wastes subjected to different treatments at the same pH
and temperatures showed that the virus inactivation in the mixed waste
s was related, at least in part, to microbial activity. In mixed STE a
nd DCMS, the D values at 25 degrees C were 8.3 for raw mixed wastes, 1
5.1 for autoclaved mixed wastes, and 9.6 for bacterium-free filtrate o
f raw mixed wastes; D values at 37 degrees C were 6.8, 10.1, and 7.0 f
or these three suspension media, respectively. In mixed STE and SMS, t
he D values at 25 degrees C were 8.1 for raw mixed wastes, 14.3 for au
toclaved mixed wastes, and 9.1 for bacterium-free filtrate of raw mixe
d wastes; the D values at 37 degrees C were 6.8, 9.4, and 6.9 for the
three suspensions, respectively.