A. Dalsgaard et al., APPLICATION OF RIBOTYPING FOR DIFFERENTIATING VIBRIO-CHOLERAE NON-01 ISOLATED FROM SHRIMP FARMS IN THAILAND, Applied and environmental microbiology, 61(1), 1995, pp. 245-251
A collection of 143 Vibrio cholerae non-01 strains isolated from shrim
p farms in Thailand were characterized and grouped by ribotyping. Sixt
y-four ribotypes were distinguished following digestion of chromosomal
DNA with the restriction enzyme BglI, and the reproducibility of the
method was 100%. There was no correlation between specific ribotype di
stributions and the locations of the shrimp farms. Rihotype similarity
was examined by cluster analysis, and two main groups with 10 and 54
ribotypes, respectively, were found. Correlation between ribotype and
O-antigen expression was shown to exist among those isolates tested. R
ibotyping appears to be a suitable method for differentiating environm
ental V. cholerae non-O1 strains, and comparison of ribotype patterns
showed a high degree of genetic divergence within V. cholerae non-O1.