IDENTIFICATION OF A PUTP PROLINE PERMEASE GENE HOMOLOG FROM STAPHYLOCOCCUS-AUREUS BY EXPRESSION CLONING OF THE HIGH-AFFINITY PROLINE TRANSPORT-SYSTEM IN ESCHERICHIA-COLI

Citation
Pa. Wengender et Kj. Miller, IDENTIFICATION OF A PUTP PROLINE PERMEASE GENE HOMOLOG FROM STAPHYLOCOCCUS-AUREUS BY EXPRESSION CLONING OF THE HIGH-AFFINITY PROLINE TRANSPORT-SYSTEM IN ESCHERICHIA-COLI, Applied and environmental microbiology, 61(1), 1995, pp. 252-259
Citations number
42
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
00992240
Volume
61
Issue
1
Year of publication
1995
Pages
252 - 259
Database
ISI
SICI code
0099-2240(1995)61:1<252:IOAPPP>2.0.ZU;2-D
Abstract
The important food-borne pathogen Staphylococcus aureus is distinguish ed by its ability to grow at low water activity values. Previous work in our laboratory and by others has revealed that proline accumulation via transport is an important osmoregulatory strategy employed by thi s bacterium, Furthermore, proline uptake by this bacterium has been sh own to be mediated by two distinct transport systems: a high-affinity system and a low-affinity system (J.-H. Bae, and K. J. Miller, Appl. E nviron. Microbiol. 58:471-475, 1992; D. E. Townsend and B. J. Wilkinso n, J. Bacteriol. 174:2702-2710, 1992). In the present study, we report the cloning of the high-affinity proline transport system of S. aureu s by functional expression in an Escherichia coli host. The sequence o f the staphylococcal proline permease gene was predicted to encode a p rotein of 497 amino acids which shares 49% identity with the PutP high -affinity proline permease of E. coli. Analysis of hydropathy also ind icated a common overall structure for these proteins.