DETECTION OF ESCHERICHIA-COLI O157-H7 IN MEAT BY AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY, EHEC-TEK

Authors
JOHNSON RP DURHAM RJ JOHNSON ST MACDONALD LA JEFFREY SR BUTMAN BT
Citation
Rp. Johnson et al., DETECTION OF ESCHERICHIA-COLI O157-H7 IN MEAT BY AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY, EHEC-TEK, Applied and environmental microbiology, 61(1), 1995, pp. 386-388
Citations number
12
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
1
Year of publication
1995
Pages
386 - 388
Database
ISI
SICI code
0099-2240(1995)61:1<386:DOEOIM>2.0.ZU;2-J
Abstract
Investigation of the specificity of an enzyme-linked immunosorbent ass ay (ELISA) for detection of Escherichia coli O157:H7 in raw meats (EHE C-Tek; Organon Teknika Corp.) revealed that the target antigens of the detection reagent; monoclonal antibody 4E8C12, were present in numero us serotypes of E. coli and that their ELISA reactivity was influenced by bile salts, acriflavine, and heat. The specificity of the ELISA wa s improved by a modified test protocol incorporating immunocapture.